Samples were prepared according to standardized approaches for synchrotron SAXS data collection44 (link).
The RBD from Spike glycoprotein, SARS-CoV-2, was expressed in HEK293-F cells and purified as previously described37 (link). Purified RBD was stored frozen (−80 °C) and dialyzed overnight into a PBS buffer before measurement.
Standard proteins were purchased from Sigma (Darmstadt, Germany): Bovine Thyroglobulin (Sigma-T1001), Equine Apoferritin (Sigma-A3660), and Bovine Serum Albumin (Sigma-A7030). Each standard was dissolved in a PBS buffer and filtered using an Ultrafree®-MC spin-filter device (12,000×g, 4 min.) prior to measurement.
The RBD from Spike glycoprotein, SARS-CoV-2, was expressed in HEK293-F cells and purified as previously described37 (link). Purified RBD was stored frozen (−80 °C) and dialyzed overnight into a PBS buffer before measurement.
Standard proteins were purchased from Sigma (Darmstadt, Germany): Bovine Thyroglobulin (Sigma-T1001), Equine Apoferritin (Sigma-A3660), and Bovine Serum Albumin (Sigma-A7030). Each standard was dissolved in a PBS buffer and filtered using an Ultrafree®-MC spin-filter device (12,000×g, 4 min.) prior to measurement.
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