Cloning and Sequencing of Potato FtsZ1

S. tuberosum var. ‘Desirée’ were grown in Magenta GA7 boxes with MS Reg media [48 (link)]. RNA was extracted from ~1-month-old tissue using TRI Reagent, as per the manufacturer’s instructions (Molecular Research Center, Cincinnati, OH, USA). RNA was cleaned with a Zymo Research RNA Clean and Concentrator kit (Irvine, CA, USA). cDNA was synthesized according to protocol using a Thermo Fisher Scientific SuperScript III First-Strand Synthesis System (Waltham, MA, USA). The FtsZ1 coding sequence was amplified using primers 1 and 2 (all primers used in this study can be found in Supplementary Table S3). The FtsZ1 amplicon and pUC19 were digested with XbaI and HindIII (NEB, Ipswich, MA, USA). One microliter of calf intestinal alkaline phosphatase was added to the pUC19 digestion for dephosphorylation, and the amplicon was ligated into the vector. The vector was Sanger sequenced with M13 forward and reverse primers.