Stains used in this study are presented Table 1 . C. jejuni strains were stored at -80°C in Brain Heart Infusion broth (BHI) containing 20% (vol/vol) glycerol. Prior to each experiment frozen cells were streaked on Karmali agar plates (Oxoid Limited, UK), incubated at 42°C for 24 h under microaerobic conditions in CampyGen sachet (Oxoid Limited, UK): 5% oxygen, 10% carbon dioxide, and 85% nitrogen.
As described previously by Rodrigues et al. (2015) (link), C. jejuni Bf cells can be acclimated to aerobic conditions (namely AAC cells for aerobically acclimated cells). This was performed by sub-culturing three times (once for 48 h and then twice 24 h) on Karmali agar plates under aerobiosis (air; Rodrigues et al., 2015 (link)). In order to maintain the same conditions for all samples, cultures under microaerobiosis were identically performed three times under microaerobiosis (MAC cells for microaerobic conditions).
As described previously by Rodrigues et al. (2015) (link), C. jejuni Bf cells can be acclimated to aerobic conditions (namely AAC cells for aerobically acclimated cells). This was performed by sub-culturing three times (once for 48 h and then twice 24 h) on Karmali agar plates under aerobiosis (air; Rodrigues et al., 2015 (link)). In order to maintain the same conditions for all samples, cultures under microaerobiosis were identically performed three times under microaerobiosis (MAC cells for microaerobic conditions).
Free full text: Click here
