Stable Cell Line Generation for Cancer Research

The parental ID8 cell line was obtained from Katherine Roby (University of Kansas Medical Center, Kansas City, Kansas, USA). ID8 cells that stably express RFP or turboGFP (tGFP) have been previously described (7 (link)). The parental MC-38 cell line was obtained from Ronald DePinho (University of Texas MD Anderson Cancer Center) with permission from Jeffrey Schlom (National Cancer Institute). ID8 and MC-38 cells were cultured in DMEM supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin (GenDEPOT). To generate MC-38 cells that stably express tGFP, MC-38 cells were transfected with pGFP-V-RS vector (Origene) by using Lipofectamine 3000 reagent (Invitrogen), followed by selection with 2 μg/mL puromycin (Sigma-Aldrich).