Keratinocyte Isolation from Atopic Dermatitis Skin
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Corresponding Organization :
Other organizations : University of Iowa, University of British Columbia, BC Cancer Agency
Protocol cited in 3 other protocols
Variable analysis
- Skin punch biopsies from buttocks region of AD DC and normal donors
- Keratinocyte isolation and culture
- Sex and age (within 1 year) matched normal biopsies (N-HSK-1 and N-HSK-2)
- Passage of cells by differentially removing feeder fibroblasts with low percent trypsin/EDTA followed by complete trypsinization of remaining epithelial cells
- Passage of cells into keratinocyte serum free media (KSFM) for two passages to remove feeders before collection of cells for certain procedures
- Not explicitly mentioned
- Not explicitly mentioned
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