All animal procedures for these experiments were approved by NIAMS Animal Care and Use Committee. Sample size was chosen based on our previous work with other inhibitors of NET formation performed in animal models12 (link), 13 . Lupus-prone female MRL/MpJ-Faslpr/J mice (stock #000485, n= 10 / group) were purchased from The Jackson Laboratories. At 11 weeks of age, Alzet osmotic pumps (Alzet model 2006, Cupertino, CA), filled with MitoTEMPO (Sigma; daily dosage of 1.5 mg/kg in saline, half of the mice selected by cage) or with vehicle (half of the mice selected by cage) were implanted subcutaneously in the dorsal area of mice. Assessments were performed in a non-blinded manner and randomization to treatment versus vehicle was based on cage. After 7 weeks, mice were euthanized (18 weeks of age). Retro-orbital bleedings and urine collection were performed when mice were 10, 15, 17 and 18 weeks of age.
Protocol detail
Mitochondrial Reactive Oxygen Scavenger Therapy in Lupus Mice
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Animal
Bleedings
Female
Inhibitors
Lupus
Mice
Mitotempo
Net formation
Osmotic
Saline
Urine collection
Corresponding Organization :
Other organizations : University of Washington, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, National Institute of Allergy and Infectious Diseases
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Variable analysis
independent variables
- Treatment with MitoTEMPO (1.5 mg/kg in saline) or vehicle
- Disease progression in lupus-prone female MRL/MpJ-Faslpr/J mice
- Measured outcomes at 10, 15, 17, and 18 weeks of age (retro-orbital bleedings and urine collection)
- Lupus-prone female MRL/MpJ-Faslpr/J mice (stock #000485)
- Age of mice (11 weeks at the start of the experiment, 18 weeks at the end)
- Alzet osmotic pumps (Alzet model 2006) for drug/vehicle administration
- Randomization to treatment versus vehicle based on cage
- Positive control: Not explicitly mentioned
- Negative control: Mice receiving vehicle (saline) treatment
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