Screening Amyloid and Tau Pathology

Sera from mice immunized with AV-1959R/A, AV-1980R/A, and mixture of AV-1959R/A and AV-1980R/A, as well as control mice injected with Advax^CpG only, were screened for the ability to bind to human Aβ plaques or/and tau tangles using 40-μm brain sections of formalin-fixed cortical tissues from a severe AD case (received from Brain Bank and Tissue Repository, MIND, UC Irvine) using immunohistochemistry, as described previously [20 (link)–22 (link)]. In addition, brain sections were stained with anti-Aβ (beta-amyloid (1–42), 1:250, Invitrogen, CA) and humanized anti-tau (Armanezumab, 1:1000; Institute for Molecular Medicine, CA) antibody as positive controls. Sections were imaged using an Olympus FX1200 confocal microscope, with identical laser and detection settings across a given immunolabel.