Characterization of MPNST Cell Lines

One HSC line and two MPNST cell lines (sNF02.2, sNF96.2) were purchased from ATCC (American Type Culture Collection, ATCC). Five MPNST cell lines (ST88-14, STS26T, T265, S462, and S462TY) were kindly granted by Prof. Vincent Keng (Li X.X. et al., 2018 (link)) and Prof. Jilong Yang (Du et al., 2013 (link)). All MPNST cell lines were derived from NF1 patients, except STS26T. Cell lines were maintained in DMEM, 10% FBS and penicillin/streptomycin (Gibco, United States) and were tested mycoplasma negative every 3 months. Verification of cell lines was confirmed by Short Tandem Repeat DNA profiling (Applied Biological Materials Inc., Canada).

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Ren J.Y., Gu Y.H., Cui X.W., Long M.M., Wang W., Wei C.J., Gu B., Zhang H.B., Li Q.F, & Wang Z.C. (2020). Protein Tyrosine Phosphatase Receptor S Acts as a Metastatic Suppressor in Malignant Peripheral Nerve Sheath Tumor via Profilin 1-Induced Epithelial-Mesenchymal Transition. Frontiers in Cell and Developmental Biology, 8, 582220.

Publication 2020

sNF02.2 sNF96.2 DMEM penicillin/streptomycin Short Tandem Repeat DNA profiling

Biological Cell lines Mpnst Mycoplasma Patients Penicillin Short tandem repeat Streptomycin

Corresponding Organization : Shanghai Jiao Tong University

Other organizations : Shanghai Institute of Nutrition and Health

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Variable analysis

independent variables

Cell lines (One HSC line and two MPNST cell lines (sNF02.2, sNF96.2) from ATCC, and five MPNST cell lines (ST88-14, STS26T, T265, S462, and S462TY) from Prof. Vincent Keng and Prof. Jilong Yang)

dependent variables

Not explicitly mentioned

control variables

Cell culture conditions (DMEM, 10% FBS, penicillin/streptomycin)
Mycoplasma testing (every 3 months)
Cell line verification (Short Tandem Repeat DNA profiling)

controls

Positive control: Not specified
Negative control: Not specified

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