Drosophila Model of Alzheimer's Disease

Fly crosses were conducted and maintained at 25 °C, ~60% humidity in diurnally controlled environments. Crosses were conducted in standard cornmeal media. On the day that adults eclosed from the pupal case, they were switched to instant fly media supplemented with vehicle (ultrapure water) or with final 1 mg/mL D-520 dissolved in ultrapure water. Flies were maintained in media for 14 days, with food changed every 3–4 days. On day 14, fly heads were dissected and GFP fluorescence was examined and photographed with an Olympus BX53 microscope. Fluorescence from fly eyes was then quantified and tabulated as described before^{37 (link),44 (link)}. Aβ_1–42 stock was #32038 from Bloomington Stock center. Ctrl flies had the GMR-Gal4 driver and UAS-mCD8-GFP on the w¹¹¹⁸ background. GMR-Gal4 was #8121 from Bloomington stock center and UAS-CD8-GFP was stock #5137 from Bloomington stock center. UAS-mCD8-GFP and GMR-Gal4 were maintained as a trans-heterozygous stock to cross to w¹¹¹⁸ or UAS-Aβ_1–42.