CDK6 and β-actin protein expression was performed as previously described [14 (link)]. The primary antibody for TCEA3 was purchased from ProteinTech (ProteinTech Group, Wuhan, China). GAPDH primary antibody was purchased from Abcam (Abcam, USA).
Protocol detail
Western Blot Protein Expression Analysis
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Actin
Antibody
Cdk6
Gapdh
Protein
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Variable analysis
independent variables
- CDK6 protein expression
- β-actin protein expression
- TCEA3 protein expression
- GAPDH protein expression
- Protein expression procedures (as previously described [14])
- Positive control: Not specified
- Negative control: Not specified
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