Multimodal Microscopic Imaging of Murine Samples

Bright-field images of dissected mice and tissue samples were captured using a Leica MZ10 F fluorescence stereomicroscope equipped with a 0.7× C-mount, Achromat 1.0× 90 mm objective, a SOLA light engine, a DS-Fi1 color camera (Nikon), and NIS Elements Basic Research software (Nikon), as described previously (107 (link), 108 (link)). H&E-stained sections were imaged using a Nikon Eclipse 80i microscope equipped with CFI Plan Fluor 4×, 10×, 20×, and 40× objective lenses; a DS-Fi1 color camera; and NIS-Elements Basic Research software. Immunofluorescently stained slides were also imaged using Plan-Apochromat objectives with DIC (63×/1.4 oil, and 20×/0.8) on a Zeiss LSM800 Airyscan inverted digital spectral confocal microscope equipped with Definite Focus 2.0; 3 confocal GaAsP detectors, including Airyscan detector with 6000 × 6000 pixels resolution; and solid-state laser module with a 405, 488, 561, and 640 nm beam splitter. Image stacks of vertical projections were assembled using Zeiss Zen 2.5 imaging software.