To prepare the recharging solutions, CPP-ACP paste (Tooth Mousse, GC, Tokyo, Japan) (S1 Table) and deionized water were used at a ratio of 1:3 [33 (link)]. Each 5 ml of deionized water was stirred with 1.7 g CPP-ACP in a vortex machine (ZX3 Vortex Mixer, VELP Scientifica, Usmate Velate MB, Italy) at a power setting of 3 for 1 min [26 (link)]. Tooth Mousse contains 10% CPP-ACP with 325 mM calcium and 187 mM phosphate [34 (link), 35 ].
Free full text: Click here