Tissues and cells were lysed with a modified buffer, and western blotting was performed as described previously41 (link). The primary antibodies were as follows: MLXIPL (Abcam, ab92809), GLUT1 (Abcam, ab115730), PKM1 (Abcam, ab116271), PKM2 (Abcam, ab137852), LDHA (Abcam, ab84716), and β-actin (Abbkine, A01011). And images were captured using an Amersham Imager 600 System (GE Healthcare).
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