F. graminearum Protein Extraction and Analysis

F. graminearum protein extraction was performed, as described previously [41 (link)]. The resulting proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilon-P transfer membrane (Millipore, Billerica, MA, USA). GFP tagged proteins were detected with monoclonal anti-GFP (ab32146, Abcam, Cambridge, UK) antibody; the GAPDH level was detected by the monoclonal anti-GAPDH antibody (Huabio M1306-4) for protein loading reference. Each experiment was repeated three times.

Free full text: Click here

Ma T., Li Y., Lou Y., Shi J., Sun K., Ma Z., Yan L, & Yin Y. (2022). The Drug H+ Antiporter FgQdr2 Is Essential for Multiple Drug Resistance, Ion Homeostasis, and Pathogenicity in Fusarium graminearum. Journal of Fungi, 8(10), 1009.

Publication 2022

Immobilon-P transfer membrane ab32146

Anti antibody Antibody Gapdh Immobilon p Membrane Protein Sds page

Corresponding Organization : Ningbo Academy of Agricultural Sciences

Other organizations : Zhejiang A & F University

Top 5 similar protocols

Variable analysis

independent variables

F. graminearum protein extraction

dependent variables

Protein separation by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
Protein transfer to Immobilon-P transfer membrane
GFP tagged proteins detected with monoclonal anti-GFP (ab32146, Abcam, Cambridge, UK) antibody
GAPDH level detected by the monoclonal anti-GAPDH antibody (Huabio M1306-4)

control variables

Protein loading reference

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!