CCI was performed as described previously (Leitner et al., 2013 (link)). Briefly, mice were deeply anesthetized with 1.25–1.5% isoflurane and the right sciatic nerve was exposed at the mid-thigh level. After removal of adherent tissue, three loose ligatures with chromic gut 6/0 (SMI) were tied 1 mm apart around the nerve, proximal to the trifurcation. Muscle and skin were closed with sutures (Silkam 6/0 and Safil 4/0, respectively; Ethicon) and wound clips.
Protocol detail
Chronic Constriction Injury of Mouse Sciatic Nerve
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Other organizations : Medical University of Vienna
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Variable analysis
independent variables
- Sciatic nerve ligation
- Not explicitly mentioned
- Anesthesia: 1.25–1.5% isoflurane
- Surgical procedure: Exposure of the right sciatic nerve at the mid-thigh level, removal of adherent tissue, and closure of muscle and skin with sutures
- Positive control: Not mentioned
- Negative control: Not mentioned
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