Fresh, healthy fallopian tube fimbriae were obtained from 3 donors at UPMC Magee-Women’s Hospital. Specimens were collected in a 50 ml of DMEM F12 Ham medium (Sigma-Aldrich) supplemented with 10% FBS (Gibco) and 1% Penn/Strep (Sigma-Aldrich). Upon receipt, FT tissues were processed under sterile tissue culture conditions as described earlier [30 (link),31 (link)] and cultured in Prigrow cell culture medium (Applied Biological Materials Inc., catalog #TM004) supplemented with 2% Ultroser G serum substitute (Pall France). To immortalize FT epithelial (FTE) cells, we used lentiviral transduction with SV40 large plus small T antigen (FTE-Tag) that suppresses TP53 signal transduction pathway thus mimicking the genetic makeup of STIC lesion [31 (link)]. Unmodified lentivirus was used in control cells. Recombinant lentiviral expression vector (Catalogue #CILV01) and an empty lentiviral expression vector (Catalogue # LV000) were commercially purchased from ALSTEM and used to transduce primary FTE cells in the presence of transduction reagent TransPlus following the manufacturer’s protocol [32 (link)–34 (link)].