Characterization of MM-specific T Cells

A flow cytometry-based, phenotypic, and functional characterization of IFNγ-producing MM-specific T cells was carried out in two selected patients with robust responses in the ELISpot assay, by applying the CSA Detection Kit (Miltenyi Biotec, Bologna, Italy), according to the manufacturer’s instructions and protocols we previously described [34 (link),35 (link)], using, as specific antigenic stimulation, the 10 MMAA-derived peptide pools, individually. T-cell phenotype and memory profile of cytokine-producing lymphocytes were assessed after sample counterstaining with monoclonal antibodies for flow cytometry, including: CD3, CD8, CD4, CD62L, and CCR7 (Miltenyi Biotec, Bologna, Italy).

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Lagreca I., Nasillo V., Barozzi P., Castelli I., Basso S., Castellano S., Paolini A., Maccaferri M., Colaci E., Vallerini D., Natali P., Debbia D., Pirotti T., Ottomano A.M., Maffei R., Bettelli F., Giusti D., Messerotti A., Gilioli A., Pioli V., Leonardi G., Forghieri F., Bresciani P., Cuoghi A., Morselli M., Manfredini R., Longo G., Candoni A., Marasca R., Potenza L., Tagliafico E., Trenti T., Comoli P., Luppi M, & Riva G. (2023). Prognostic Relevance of Multi-Antigenic Myeloma-Specific T-Cell Assay in Patients with Monoclonal Gammopathies. Cancers, 15(3), 972.

Publication 2023

CSA Detection Kit CD62L

Antigenic Assay Cd62l Cytokine Elispot Flow cytometry Ifnγ Lymphocytes Memory Monoclonal antibodies Out patients Peptide Phenotype T cell

Corresponding Organization : University of Modena and Reggio Emilia

Other organizations : Istituti di Ricovero e Cura a Carattere Scientifico, Ferrari (Italy)

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Variable analysis

independent variables

10 MMAA-derived peptide pools, individually

dependent variables

T-cell phenotype
Memory profile of cytokine-producing lymphocytes

control variables

Monoclonal antibodies for flow cytometry, including: CD3, CD8, CD4, CD62L, and CCR7

controls

Positive controls: Not explicitly mentioned.
Negative controls: Not explicitly mentioned.

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