Immunohistochemistry of Tissue Sections

For IF, paraffin-embedded sections were stained as deScribed previously^{40 (link)} using the following primary antibodies: TAZ (Cell Signaling, 1:1000), K18 (Sigma, 1:200), αSMA (Sigma, 1:250), Ki67 (Abcam, 1:1000), caspase 3 (Cell Signaling, 1:50), AQP (Calbiochem, 1:100; Darmstadt, Germany), Scrib (Santa Cruz, 1:100), Vangl2 (Santa Cruz, 1:50), GM130 (BD Bioscience, 1:150), Sox9 (Millipore, 1:100), NKCC and Slug (Santa Cruz, 1:100). Alexa 488 and Alex 568-conjugated secondary antibodies (ThermoFisher Scientific, Molecular Probes, Waltham, MA, USA) were used at 1:1000. DAPI (6-diamidino-2-phenylindole) was used to counterstain nuclei. Images were acquired using Zeiss Axiovert 40CFL microscope. For IHC, the following primary antibodies were used: vimentin (BD Bioscience, 1:200), LKB1 (Cell Signaling, 1:100), BMP4 (Abcam, 1:100), sox2 (Abcam, 1:100), and ALDH (BD Bioscience, 1:50). For detection, the ImmPRESS REAGENT KIT (Vector Laboratories, MP-7500) was used followed by the DAB kit (Vector Laboratories, SK-4100) and counterstained with hematoxylin (Vector Laboratories, H-3041).