HAp crystals were labeled via fluorexon staining. Briefly, 100mg nHAp crystals and 10 mL fluorexon solution (10mg/mL) were mixed and stirred for 30 min at room temperature. Subsequently, the HAp crystals were collected via centrifugation, washed three times with anhydrous ethanol and distilled water to make sure that there was no free fluorexon residue, and dried.
Protocol detail
Fluorexon Labeling of Hydroxyapatite Crystals
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Corresponding Organization : Second Affiliated Hospital of Zhejiang University
Other organizations : Qingdao University, Affiliated Hospital of Qingdao University
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Variable analysis
independent variables
- Amount of nHAp crystals (100mg)
- Degree of labeling/staining of HAp crystals with fluorexon
- Concentration of fluorexon solution (10mg/mL)
- Duration of mixing/stirring (30 min)
- Temperature (room temperature)
- Washing and drying procedure
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