Influenza Hemagglutinin Epitope Detection

Cell culture materials were purchased from Invitrogen, FR900359 (previous commercial name UBO-QIC) was isolated and purified as described elsewhere (24 (link)), YM-254890 was from Wako Chemicals GmbH. Primary antibodies to detect the human influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) and α-tubulin were from Roche Applied Science and LifeSpan BioSciences, respectively. The horseradish peroxidase–conjugated secondary antibodies, goat anti-mouse IgG, and goat anti-rabbit IgG were from Sigma-Aldrich and Antibodies-online GmbH, respectively. All other reagents were purchased from Sigma-Aldrich if not stated otherwise.

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Patt J., Alenfelder J., Pfeil E.M., Voss J.H., Merten N., Eryilmaz F., Heycke N., Rick U., Inoue A., Kehraus S., Deupi X., Müller C.E., König G.M., Crüsemann M, & Kostenis E. (2021). An experimental strategy to probe Gq contribution to signal transduction in living cells. The Journal of Biological Chemistry, 296, 100472.

Publication 2021

YM-254890 LifeSpan horseradish peroxidase–conjugated secondary antibodies goat anti-mouse IgG goat anti-rabbit IgG

Anti igg Antibodies Cell culture Epitope Fr900359 Goat Hemagglutinin Horseradish peroxidase Human influenza Mouse Rabbit Ubo qic Ym 254890 α tubulin

Corresponding Organization : University of Bonn

Other organizations : Tohoku University, Paul Scherrer Institute

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Variable analysis

independent variables

Cell culture materials purchased from Invitrogen
FR900359 (previous commercial name UBO-QIC) isolated and purified as described elsewhere (24 (link))
YM-254890 from Wako Chemicals GmbH

dependent variables

Not explicitly mentioned

control variables

Primary antibodies to detect the human influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) and α-tubulin from Roche Applied Science and LifeSpan BioSciences, respectively
Horseradish peroxidase–conjugated secondary antibodies, goat anti-mouse IgG, and goat anti-rabbit IgG from Sigma-Aldrich and Antibodies-online GmbH, respectively
All other reagents purchased from Sigma-Aldrich if not stated otherwise

controls

Positive control: Not specified
Negative control: Not specified

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