Oxidative Stress Parameters in Brain

For the determination of the oxidative stress parameters and antioxidant components in the brain, frozen tissues were homogenized (RIPA buffer, Thermo Scientific) supplemented with Complete and Phospho STOP (Roche, Basel, Switzerland) protease and phosphatase inhibitors), as described [18 (link)]. The activities of superoxide dismutase (SOD) and catalase were studied with commercially available kits (CA061 and CA063, respectively, Canvax, Córdoba, Spain). Lipid peroxidation was determined using a commercial TBARS assay kit (CA995, Canvax). The final products were detected with a microplate reader (POLARstar Omega, BMG Labtech, Ortenberg, Germany) at 530/590 nm (for catalase and TBARS) or 450 nm for SOD.