Routine Culturing and Motility Assays of M. xanthus

M. xanthus strains were cultured according to previously established protocols, primarily using CYE liquid media for routine culturing and 0.5% agar CYE for motility assays. M. xanthus strains utilized that were all reported previously: cglB- (Rodriguez and Spormann, 1999 (link)), epsZ- (Berleman et al., 2011 (link)), pilT- (Wu et al., 1997 (link)) pilA::gfp (Bustamante et al., 2004 (link)). Environmental isolates of M. xanthus were enriched using previously described methods (Vos and Velicer, 2008 (link)) harvesting small quantities of local soils as a source for new strains. Strains were purified for isolation through routine restreaking on CYE and confirmation of species identification of each isolate was determined through 16S rDNA sequencing. Examination of each isolate for S-motility patterns was performed using a Nikon SMZ1500 stereo microscope (Nikon Instruments Inc., Melville, NY, USA)

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Berleman J.E., Zemla M., Remis J.P., Liu H., Davis A.E., Worth A.N., West Z., Zhang A., Park H., Bosneaga E., van Leer B., Tsai W., Zusman D.R, & Auer M. (2016). Exopolysaccharide microchannels direct bacterial motility and organize multicellular behavior. The ISME Journal, 10(11), 2620-2632.

Publication 2016

Nikon SMZ1500 stereo microscope

Agar Culturing media Isolation Microscope Motility Motility assays Rdna Strains

Corresponding Organization : University of California, Berkeley

Other organizations : Shandong University

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Variable analysis

independent variables

M. xanthus strains: cglB-, epsZ-, pilT-, and pilA::gfp
Environmental isolates of M. xanthus

dependent variables

Motility patterns of M. xanthus strains

control variables

CYE liquid media for routine culturing
0.5% agar CYE for motility assays
Nikon SMZ1500 stereo microscope for examining motility patterns

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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