Amygdala Injection and Seizure Scoring

As previously reported [11 (link)], mice with guide cannulas were gently restrained and an infusion cannula (IO: 0.3 mm; RWD Life Science.Inc) was inserted into the amygdala through the guide cannula. 0.15 l of KA (3 mg/ml) was infused into the amygdala at the flow rate of 2 nl/s controlled by microinjector (NanojectIII, Drummond Scientific). The cannula was kept in the right amygdala for two additional min after completion of infusion and withdrew slowly to minimize reflux along the injection tract. Behavioral seizures were classified based on the criteria described by Racine[43 (link)] and scored every 5 min by a blinded investigator: stage 0, no seizure; stage 1, arrest and rigid posture; stage 2, head nodding; stage 3, sporadic full-body shaking, spasms; stage 4, chronic full-body spasms; stage 5, jumping, shrieking, falling over; stage 6, violent convulsions or death.

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Wang J., Huang J., Yao S., Wu J.H., Li H.B., Gao F., Wang Y., Huang G.B., You Q.L., Li J., Chen X, & Sun X.D. (2021). The ketogenic diet increases Neuregulin 1 expression via elevating histone acetylation and its anti-seizure effect requires ErbB4 kinase activity. Cell & Bioscience, 11, 93.

Publication 2021

infusion cannula NanojectIII

Amygdala Body Cannula Convulsions Head Mice Rigid Spasms

Corresponding Organization :

Other organizations : Second Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Jinan University, Guangdong Province Women and Children Hospital

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Variable analysis

independent variables

Infusion of KA (3 mg/ml) into the amygdala

dependent variables

Behavioral seizures classified based on Racine scale (stage 0 to stage 6)

control variables

Mice with guide cannulas
Infusion cannula (I.D.: 0.3 mm) inserted into the amygdala through the guide cannula
Infusion rate of 2 nl/s controlled by microinjector
Cannula kept in the right amygdala for two additional minutes after completion of infusion to minimize reflux
Behavioral seizures scored every 5 minutes by a blinded investigator

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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