Modulation of Mx1-Cre Induction Pathways

Poly I:C (Invivogen) was administered intraperitoneally (i.p.) every other day at 5 mg/kg for three doses for Mx1-Cre induction. NAC (Sigma #A7250) was administered at 50 mg/kg in PBS i.p. daily and supplemented at 1 mg/ml via drinking water which was refreshed twice a week. CFZ (CFZ/PR-171, Selleckchem), rapamycin (sirolimus, Selleckchem) and verapamil (Sigma #V4629) were dissolved in ethanol first and then diluted in 1% PEG400/1%Tween80/PBS for injections. CFZ was injected intravenously at 2 mg/kg on days 1 and 2 of each week. rapamycin was administered at 4 mg/kg i.p. daily and supplemented at 15 ng/ml in drinking water when specified^{50 (link)}. RTKi PKC412 (Midostaurin) was purchased from Selleck Chemicals (Cat# S8064) and injected i.p. daily at 3 mg/kg. LiCl^{40 (link)} (1 mEq/kg, Fisher #L121) and verapamil^{41 (link)} (25 mg/kg, Sigma #V4629) were also injected i.p. daily. All drugs used for rescue purpose were administered starting the day after the pIpC induction and throughout the course until the day of analysis.