Anti-ALK IHC was performed using the iAEP method (ALK Detection Kit, Nichirei Bioscience, Tokyo, Japan) with an automatic staining protocol13 (link),25 (link). To confirm ALK positivity, IHC (and/or FISH) was also performed and evaluated by a commercial clinical laboratory (SRL, Tokyo). ALK IHC results were classified using iScore for iAEP IHC14 (link),26 (link).
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