Maintenance and Differentiation of Human iPSCs

The human iPSC line Ff-I01s04 was provided by Kyoto University. Accession Number of Ff-I01s04 is ‘CVCL_C1DY’ in Cellosaurus of Expasy (Swiss Bioinfomatics Resource Portal). As we previously reported [14 (link)], all iPSC lines were maintained using the dish coated by iMatrix-511 silk (Nippi, Tokyo, Japan) and StemFit AK02N medium (ReproCELL, Yokohama, Japan). The medium was changed at day 1, 3, 5, and 6; the detachment for passage and differentiation was performed using accutase (Innovative Cell Technologies, San Diego, CA, USA) at day 7. The use of human iPSCs was approved by the ethics committee of The Institute of Medical Science, The University of Tokyo (Approval Code: 2019-4-0716).