Vero cells (ECACC 84113001) were cultured in Nunc cell-culture flasks of 75 cm2 surface area (Thermo Fischer Scientific, Waltham, MA, USA) in Minimum Essential Medium (MEM) (Sigma Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Gibco, Waltham, MA, USA) previously inactivated at 56 °C for 30 min (iFBS), plus antibiotics (penicillin 100 U/mL, streptomycin 100 μg/mL). The cultures were maintained at 37 °C, in a moist atmosphere enriched with 5% CO2.
Trypanosoma cruzi Pan4 strain (TcIa + TcId) was employed in this work. This strain was isolated from a patient from Panama during 2004. The epimastigote stage of the parasite was cultured in liver infusion tryptose (LIT) medium supplemented with 10% heat-inactivated fetal bovine serum, at 28 °C. Trypomastigote forms derived from cell cultures were obtained from infected Vero cell cultures as previously described [51 (link),52 (link),53 (link),54 (link)].
Trypanosoma cruzi Pan4 strain (TcIa + TcId) was employed in this work. This strain was isolated from a patient from Panama during 2004. The epimastigote stage of the parasite was cultured in liver infusion tryptose (LIT) medium supplemented with 10% heat-inactivated fetal bovine serum, at 28 °C. Trypomastigote forms derived from cell cultures were obtained from infected Vero cell cultures as previously described [51 (link),52 (link),53 (link),54 (link)].
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