Cytotoxic Drugs and Inhibitor Preparation

Drugs: VP-16, CDDP, and two inhibitors, A12B4C3 and DDRI-18, were obtained from Sigma-Aldrich (St. Louis, MO, USA), while the third inhibitor—YU238259—was synthesized using TriMen Chemicals (Lodz, Poland) as previously described [11 (link)]. VP-16 was dissolved in methanol in the 10 mM stock solution. CDDP was dissolved in water in the 5 mM stock solution. A12B4C3, DDRI-18, and YU238259 were prepared as 50 mM stock solutions in anhydrous dimethylsulfoxide (DMSO). The chemical structure of the drugs and inhibitors is shown in Figure 2. Other chemicals: Normal melting point (NMP) and low melting point (LMP) agarose, Triton X-100, Tris HCl, phosphate-buffered saline (PBS), NaCl, NaHCO3, NaOH, ethylenediaminetetraacetic acid (EDTA), 4′,6-diamidino-2-phenylindole (DAPI), propidium iodide (PI), DNase-free RNAse were obtained from Sigma-Aldrich (St. Louis, MO, USA).