Sperm Motility Analysis in TYH Medium

Sperm motility analysis was conducted as described previously (Miyata et al., 2021 (link)). Cauda epididymal spermatozoa were suspended and incubated in TYH medium, which can induce sperm capacitation (Toyoda and Yokoyama, 2016 (link)). Sperm motility was then measured using the CEROS II sperm analysis system (software version 1.5; Hamilton Thorne Biosciences). The motility of epididymal spermatozoa was recorded after 10 min and 2 h of incubation in TYH medium.

Free full text: Click here

Shimada K, & Ikawa M. (2023). CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility. Development (Cambridge, England), 150(21), dev201724.

Publication 2023

CEROS II sperm analysis system

Cauda Epididymal Motility spermatozoa Sperm Sperm capacitation

Corresponding Organization :

Other organizations : Osaka University, The University of Tokyo

Top 5 similar protocols

Variable analysis

independent variables

Incubation time of epididymal spermatozoa in TYH medium (10 min and 2 h)

dependent variables

Sperm motility

control variables

Cauda epididymal spermatozoa
TYH medium (can induce sperm capacitation)
CEROS II sperm analysis system (software version 1.5)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!