Characterization of NSCLC Cell Lines

The human NSCLC cell lines A549, NCI-H1299, NCI-H23, NCI-H3255, NCI-H1650, and HCC-827 were purchased from ATCC (Manassas, VA, USA) and cultured as recommended. HCC-827GR5 and PC9 were a kind gift from Dr. Pasi A. Jänne, Harvard University, Boston, MA [15 (link)]. Cells were grown as a monolayer in 75 cm² flasks (Costar, Cambridge, MA, USA) at 37 °C in 5% CO₂ and 95% air. The NSCLC cell lines used in this study had been previously characterized for EGFR and KRAS mutations, and were tested for their authentication by PCR profiling using short tandem repeats (STR), at BaseClear (Leiden, The Netherlands).

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Leonetti A., Capula M., Minari R., Mazzaschi G., Gregori A., El Hassouni B., Papini F., Bordi P., Verzè M., Avan A., Tiseo M, & Giovannetti E. (2021). Dynamic Evaluation of Circulating miRNA Profile in EGFR-Mutated NSCLC Patients Treated with EGFR-TKIs. Cells, 10(6), 1520.

Publication 2021

NCI-H1299 NCI-H23 NCI-H3255 NCI-H1650 HCC-827 75 cm2 flasks

Cell lines Cells Egfr Human Kras Mutations Nsclc

Corresponding Organization : Fondazione Pisa

Other organizations : Scuola Superiore Sant'Anna, Mashhad University of Medical Sciences

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Variable analysis

independent variables

NSCLC cell lines: A549, NCI-H1299, NCI-H23, NCI-H3255, NCI-H1650, HCC-827, HCC-827GR5, PC9

dependent variables

Not explicitly mentioned

control variables

Cell growth conditions: 75 cm^2 flasks, 37 °C, 5% CO2, 95% air
Cell line authentication: PCR profiling using short tandem repeats (STR) at BaseClear

controls

No positive or negative controls were explicitly mentioned in the input text.

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