Drosophila Genetics Protocols for Research

All flies were raised on standard food at 25 °C. UAS-(GR)₈₀ and UAS-CONT-(GR)₈₀ flies as well as UAS-(G₄C₂)₁₆₀ and UAS-(G₄C₂)₅ flies have been obtained from earlier studies^{40 (link)}. A deficiency kit for the second chromosome and ELAV-GAL4, TubGAL80^ts, UAS-p35, lilli^k05431, lilli^A17-2, UAS-GFP, UAS-lacZ, OK³⁷¹-GAL4, and GMR-GAL4 fly stocks were from the Bloomington Drosophila Stock Center. Lilli RNAi line (v106142) was from the Vienna Drosophila Resource Center (VDRC). lilli^4U5 and UAS-lilli-HA fly stocks were a gift from Dr Ernst Hafen. The lilli^A17-2 allele was created by ethyl methanesulfonate mutagenesis. The nature of the mutation is unknown but is thought to be a loss of function allele^{55 (link)}. The lilli^k05431 allele is a P-element insertion^{56 (link)}. The lilli^4U5 allele has a short deletion resulting in a frameshift and a functional null allele^{56 (link)}.