PA and Ethylene Quantification in Plants

To analyse the PA contents, the plant samples of WT, OE, osacl5 were collected. 0.3–0.5 g samples per plant were pulverised and the PAs were extracted following a previous method (Liu et al., 2014a (link)). The benozylated PAs were assayed with a programmable Agilent HPLC using a reverse‐phase column (ZORBAX SB‐C18 4.6 × 250 mm, PACKING LOT #: B17225, US), and detected at 254 nm. The ethylene evolution was measured as described previously (Tao et al., 2018 (link)). The gas chromatograph (Model GC‐17A; Shimadzu Co., Kyoto, Japan) fitted with a flame ionisation detector and an activated alumina column (200 cm × 0.3 cm) with an injector temperature of 120 °C, column temperature of 60 °C, and detector temperature of 60 °C as described previously (Shan et al., 2012 (link)).