IL-1β (211-11B; PeproTech, Rocky Hill, NJ, USA) was dissolved in DKSFM to prepare stocks with a concentration of 10 mg/mL. Rosiglitazone (HY-17386; MedChemExpress, Monmouth, Junction, NJ, USA) was dissolved in DKSFM to prepare stocks with a concentration of 5 mM. HMGDCs were exposed to medium containing 50 ng/mL IL-1β, 50 ng/mL IL-1β + 50 µM Rosiglitazone, or DKSFM alone for a duration of 48 hours, when the cells reached approximately 60% confluency. These concentrations of pharmacologic reagents were selected based on previous studies conducted on mouse MG explants. The earlier investigations revealed that the treatment of mice MGs with 50 ng/mL IL-1β did not yield any noteworthy impact on cell activity, while a noticeable keratinizing effect was observed.8 ,9 (link)
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Effect of IL-1β and Rosiglitazone on HMGDCs
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Corresponding Organization : Huazhong University of Science and Technology
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Variable analysis
independent variables
- IL-1β concentration (50 ng/mL)
- Rosiglitazone concentration (50 µM)
- Cell activity
- Cell confluency (approximately 60%)
- Duration of treatment (48 hours)
- Positive control: Cells exposed to 50 ng/mL IL-1β
- Negative control: Cells exposed to DKSFM alone
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