Western Blotting of Liver Proteins

Western blotting analyses on whole liver protein samples were performed as previously described [31 (link)]. The separated proteins on the PVDF membranes were detected using the following antibodies: mouse monoclonal antibodies against ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (anti-Uqcrfs1 [5A5] ab14746), ubiquinol-cytochrome C reductase core protein I (anti-Uqcrc1 [16D10AD9AH5] ab110252), ubiquinol-cytochrome C reductase core protein II (anti-Uqcrc2 [13G12AF12BB11] ab14745) from Abcam (Cambridge, MA, USA) and mouse monoclonal antibody β-actin (A5441) from Sigma-Aldrich (Oakville, ON, Canada).

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Aumailley L., Bodein A., Adjibade P., Leclercq M., Bourassa S., Droit A., Mazroui R, & Lebel M. (2024). Combined transcriptomics and proteomics unveil the impact of vitamin C in modulating specific protein abundance in the mouse liver. Biological Research, 57, 26.

Publication 2024

ab14746 ab110252 ab14745 β-actin (A5441)

Corresponding Organization :

Other organizations : Université Laval

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Protein expression levels of Uqcrfs1, Uqcrc1, and Uqcrc2 in whole liver protein samples

control variables

Not explicitly mentioned

controls

Positive control: β-actin antibody used to detect β-actin protein expression as a loading control

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