Native MS Analysis of SERPIN-Protease Complexes

SERPINs and proteases were stored on ice, mixed, and loaded into gold-coated needles prepared in-house and analyzed by native MS on a modified Orbitrap Eclipse Tribrid mass spectrometer in intact protein detection mode (Thermo Fisher Scientific).²² (link) Briefly, ion transmission was tuned for high m/z ions, higher-energy collisional dissociation (HCD) collision energies were optimized in the ion routing multipole for higher charge state species and the ion trap was optimized to trap, isolate and activate high m/z ions (up to 8,000 m/z). Typical MS settings were spray voltage 1.2 kV, and source temperature 150°C. The source fragmentation voltage was kept at 0–50 V to minimize in-source dissociation of glycoprotein complexes.⁵⁰ (link)

Free full text: Click here

Wu D., Guo M, & Robinson C.V. (2023). Connecting single-nucleotide polymorphisms, glycosylation status, and interactions of plasma serine protease inhibitors. Chem, 9(3), 665-681.

Publication 2023

Orbitrap Eclipse Tribrid

Corresponding Organization : University of Oxford

Top 5 similar protocols

Variable analysis

independent variables

Mixing SERPINs and proteases

dependent variables

Analysis of SERPINs and proteases by native MS on a modified Orbitrap Eclipse Tribrid mass spectrometer in intact protein detection mode

control variables

SERPINs and proteases stored on ice
Gold-coated needles prepared in-house
Ion transmission tuned for high m/z ions
Higher-energy collisional dissociation (HCD) collision energies optimized in the ion routing multipole for higher charge state species
Ion trap optimized to trap, isolate and activate high m/z ions (up to 8,000 m/z)
Spray voltage 1.2 kV
Source temperature 150°C
Source fragmentation voltage kept at 0–50 V to minimize in-source dissociation of glycoprotein complexes

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!