CMT167 cells (12 (link)) were stably transfected with firefly luciferase as previously described (11 (link)). Lewis Lung Carcinoma Cells (LL/2) were purchased from ATCC and luciferase-expressing Lewis Lung Carcinoma cells (LLC) were purchased from Caliper Life Sciences (LL/2-luc-M38). All cell lines were periodically tested for mycoplasma infection and were last retested in February 2017. To avoid cross-contamination and phenotypic changes, cells were maintained as frozen stocks and cultured for only two to four weeks before use in experiments. Authentication of cell lines based on morphology, growth curve analysis, and metastatic phenotype was performed regularly, and no phenotypic changes were observed through the duration of the study.
Protocol detail
Generating Stable Luciferase-Expressing Cell Lines
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Cell lines
Cell lines authentication
Cells
Firefly luciferase
Frozen
Lewis lung carcinoma
Luciferase
Mycoplasma infection
Phenotypic
Corresponding Organization : Veterans Health Administration
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Variable analysis
independent variables
- Stable transfection of CMT167 cells with firefly luciferase
- Luciferase expression in CMT167 cells
- Luciferase expression in Lewis Lung Carcinoma (LL/2) cells
- Metastatic phenotype of cell lines
- Maintenance of cells as frozen stocks and culturing them for only two to four weeks before use in experiments to avoid cross-contamination and phenotypic changes
- Regular authentication of cell lines based on morphology, growth curve analysis, and metastatic phenotype to ensure no phenotypic changes
- Luciferase-expressing Lewis Lung Carcinoma (LLC) cells purchased from Caliper Life Sciences
- Non-transfected CMT167 cells and LL/2 cells
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