In Vitro Transcription of Alu RNA

T7 promoter containing Alu expression plasmid was linearized and used for generating in vitro transcribed Alu RNA using AmpliScribe T7-Flash Transcription Kit (Epicenter) following the manufacturer’s instructions. The resulting Alu RNA was DNase treated and purified using MEGAclear (Ambion), and its integrity was monitored by gel electrophoresis^{4 (link),5 (link)}.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kerur N., Fukuda S., Banerjee D., Kim Y., Fu D., Apicella I., Varshney A., Yasuma R., Fowler B.J., Baghdasaryan E., Marion K.M., Huang X., Yasuma T., Hirano Y., Serbulea V., Ambati M., Ambati V.L., Kajiwara Y., Ambati K., Bastos-Carvalho A., Ogura Y., Terasaki H., Oshika T., Kim K.B., Hinton D.R., Leitinger N., Cambier J.C., Buxbaum J.D., Kenney M.C., Jazwinski S.M., Nagai H., Hara I., West A.P., Fitzgerald K.A., Sadda S.R., Gelfand B.D, & Ambati J. (2017). cGAS drives non-canonical inflammasome activation in age-related macular degeneration. Nature medicine, 24(1), 50-61.

Publication 2017

AmpliScribe T7-Flash Transcription Kit MEGAclear

Dnase Plasmid Transcription

Corresponding Organization : University of Virginia

Other organizations : University of Kentucky, Doheny Eye Institute, University of Tsukuba, Center for Digital Image Evaluation, Icahn School of Medicine at Mount Sinai, Nagoya City University, Nagoya University, Southern California Eye Institute, University of Southern California, University of Colorado Denver, University of California, Irvine, Tulane University, Kobe University, Wakayama Medical University, Texas A&M University, University of Massachusetts Chan Medical School

Top 5 similar protocols

Variable analysis

independent variables

Linearized T7 promoter containing Alu expression plasmid

dependent variables

In vitro transcribed Alu RNA

control variables

Manufacturer's instructions for AmpliScribe T7-Flash Transcription Kit (Epicenter) were followed

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!