For the measurement of incorporated miRNAs into RISC in PDAC cells, we applied Ago2 immunoprecipitation by using a microRNA Isolation Kit, Human Ago2 (Wako Pure Chemical Industries, Ltd., Osaka, Japan). The number of Ago2-conjugated miRNAs were assessed by qRT-PCR assay. The experimental procedure has been described in previous studies [32 (link),33 (link),41 (link)].
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