HEp-2 Immunoassay for Autoantibody Detection

HEp-2 assays with corresponding autoantibodies are described in ref. 28 (link) in detail. Briefly, serum samples indicated in the figures or figure legends were diluted to an equal concentration of IgM (approximately 300 ng/mL anti–Insulin-IgM in both immunized samples) and applied onto the HEp-2 slides (EUROIMMUN, F191108VA). Anti–IgM-APC (allophycocyanin) (eBioscience) and anti–IgG-APC (eBioscience) were used for the visualization of ANA-IgM/-IgG.

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Amendt T., Allies G., Nicolò A., El Ayoubi O., Young M., Röszer T., Setz C.S., Warnatz K, & Jumaa H. (2022). Autoreactive antibodies control blood glucose by regulating insulin homeostasis. Proceedings of the National Academy of Sciences of the United States of America, 119(6), e2115695119.

Publication 2022

HEp-2 slides anti–IgG-APC

Allophycocyanin Anti igg Anti igm Assays Autoantibodies Insulin Serum

Corresponding Organization : Universität Ulm

Other organizations : University Medical Center Freiburg, University of Freiburg

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Variable analysis

independent variables

Serum samples indicated in the figures or figure legends

dependent variables

Visualization of ANA-IgM/-IgG

control variables

Serum samples diluted to an equal concentration of IgM (approximately 300 ng/mL anti–Insulin-IgM in both immunized samples)
HEp-2 slides (EUROIMMUN, F191108VA)
Anti–IgM-APC (allophycocyanin) (eBioscience)
Anti–IgG-APC (eBioscience)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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