Assimilation of hydrocarbons was determined according to Satow et al. [42 (link)] and modified by Zajc et al. [3 (link)]. Liquid YNB medium (pH 7.0) was prepared without any carbon source and, after autoclaving, supplemented with 20% (v/v) mineral oil and 20% (v/v) n-hexadecane (both Sigma Aldrich, USA) as the sole carbon source. Both mineral oil and n-hexadecane were filter-sterilized before use. The test tubes were inoculated with 100 µL of cell suspension or plugs of mycelium in three replicates and incubated without shaking for one month at room temperature.
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