Single-cell RNA sequencing of lung tissue

The three Sham group specimens, the three CLP group specimens, and the three Maresin1 group specimens were mixed, respectively, for single-cell RNA sequencing. Single-cell suspensions of lung tissue specimens were prepared according to OE Biotech Co., Ltd.’s advice [22 (link)]. The cells were then sorted using Miltenyi mouse CD45 magnetic beads according to the manufacturer’s recommendations and sequenced at a 1:1 ratio. According to the manufacturer’s guidelines, the single-cell libraries were constructed by 10× Genomics Chromium Single Cell 3ʹ Reagent Kits v3.1. The Illumina Nova 6000 PE150 platform was used to sequence the libraries.