Protocol detail

CHO Cell Culture Protocol

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Chinese hamster ovary (CHO) E77.4 cells (34 (link)) purchased from ATCC were cultured in media supplemented with RPMI 1640 (Quality Biological 112-025-101), 10% heat inactivated fetal bovine serum (FBS) (Gibco 10082147), 2mM L-Glutamine (Quality Biological 118-084-721) and 100 U/mL Penicillin with 100 µg/mL Streptomycin (Quality Biological 120-095-721). Cells were passaged using 0.05% Trypsin-0.1% EDTA (Quality Biological 118-087-721). CHO cells at passages 9-12 were used for all experiments.

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Waghela I.N., Mallory K.L., Taylor J.A., Schneider C.G., Savransky T., Janse C.J., Lin P.J., Tam Y.K., Weissman D, & Angov E. (2022). Exploring in vitro expression and immune potency in mice using mRNA encoding the Plasmodium falciparum malaria antigen, CelTOS. Frontiers in Immunology, 13, 1026052.

Publication 2022

RPMI 1640 FBS L-Glutamine Penicillin Streptomycin Trypsin EDTA

Biological Cells Cells chinese hamster ovary Cultured media Edta Fetal bovine serum Glutamine Penicillin Streptomycin Trypsin 1

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Variable analysis

independent variables

Passages of CHO cells (9-12)

dependent variables

Not explicitly mentioned

control variables

Culture media (RPMI 1640, 10% heat inactivated FBS, 2mM L-Glutamine, 100 U/mL Penicillin with 100 µg/mL Streptomycin)
Cell culture conditions (passages, trypsin-EDTA solution)

controls

Negative control: Not explicitly mentioned
Positive control: Not explicitly mentioned

Annotations

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