The production of H2O2 by BSA-GOx-NPs, glucose, and oxygen was determined via a classic colorimetric method, applying ammonium titanyl oxalate as the indicator. In brief, 0.1 mL of BSA-GOx-NPs or BSA-NPs suspension (50 μg/mL BSA) was mixed with 0.1 mL of different glucose concentrations (0, 0.025, 0.05, 0.1, 0.2, 0.5, 1 and 2 mg/mL). After 1 h, ammonium titanyl oxalate solution (10 μL, 10 mM) was added. The obtained yellow suspension was measured by a microplate reader (Nano Quant, Tecan) at 405 nm.
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