Histological Analysis of PBC in China

A total of 59 paraffin-embedded liver sections, including samples from 18 HCs to 41 patients with PBC, were obtained from Xiangya Hospital (Changsha, Hunan, China) between February 2017 and September 2022. Patients with PBC were diagnosed by experienced physicians based on levels of serum alkaline phosphatase (ALP), status of serum AMAs, and histological evaluation of biopsied liver tissue. The histological sections were evaluated by experienced hepatopathologists in a blinded fashion and the stages of PBC, degree of fibrosis, and bile duct loss in each sample were scored using the Ludwig (Ludwig et al., 1978 (link)) and Nakanuma systems (Nakanuma et al., 2010 (link)). The control liver sections were obtained from normal liver tissues without unusual histological features, such as hepatic hemangioma, and nontumoral parts of livers complicated with a liver tumor. The detailed clinical characteristics of the enrolled patients are listed in Supplementary Table S1.
This study was approved by the Ethics Review Board of Xiangya Hospital Central South University (No. 20221025). Written informed consent was obtained from all participants.

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Wang H., Zhang J., Liu J., Jiang Y., Fu L, & Peng S. (2023). Identification of AKR1B10 as a key gene in primary biliary cholangitis by integrated bioinformatics analysis and experimental validation. Frontiers in Molecular Biosciences, 10, 1124956.

Publication 2023

Alkaline phosphatase Bile duct Embedded paraffin Fibrosis Hemangioma Liver tumor Livers Normal tissues Patients Physicians Serum

Corresponding Organization : Central South University

Other organizations : Second Xiangya Hospital of Central South University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Stages of PBC
Degree of fibrosis
Bile duct loss

control variables

Normal liver tissues without unusual histological features, such as hepatic hemangioma, and nontumoral parts of livers complicated with a liver tumor

controls

Positive control: Liver sections from 18 healthy controls (HCs)
Negative control: Not specified

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