RMA-S/HLA-E cells56 (link) were maintained in complete medium (RPMI-1640 containing glutamine and supplemented with 10 % (vol/vol) fetal bovine serum (FBS), 20 μM β-mercaptoethanol and 100 U ml−1 penicillin–streptomycin; all Thermo Fisher) in the presence of 400 μg ml−1 hygromycin B (InvivoGen).
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Maintenance of RMA-S/HLA-E Cells
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Corresponding Organization : Freie Universität Berlin
Other organizations : Stanford University, Max Delbrück Center, Berlin Institute of Health at Charité - Universitätsmedizin Berlin
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Variable analysis
independent variables
- Presence of 400 μg ml^-1 hygromycin B
- Maintenance of RMA-S/HLA-E cells
- Complete medium (RPMI-1640 containing glutamine and supplemented with 10 % (vol/vol) fetal bovine serum (FBS), 20 μM β-mercaptoethanol and 100 U ml^-1 penicillin–streptomycin)
- Negative control: Not explicitly mentioned
- Positive control: Not explicitly mentioned
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