Collagen I solution (Sigma-Aldrich) was added to a 96-well plate for cell adhesion detection, and 2 × 104 U251 and U87 cells were cultured into the plate at 37°C for 4 h. Post culture, the medium was discarded, and the cells were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reagent (C0009S, Beyotime, Jiangsu, China) for 2 h at 37°C. Then, 100 µL dimethyl sulfoxide was added to each well, and the OD value at 570 nm was detected using a multimode-plate-reader (Thermo Fisher Scientific) [24 (link)].
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