Immunohistochemistry analyses and β cell mass were performed as described previously (60 (link)). Images were captured using a NanoZoomer slide scanner system with NanoZoomer Digital Pathology software (Hamamatsu) or confocal fluorescence microscopy. Microscopic images were analyzed using ImageJ software.
Protocol detail
Quantitative Immunohistochemistry for β-cell Mass
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Confocal microscopy
Fluorescence
Fluorescence microscopy
Immunohistochemistry
Microscopic
Scanner
β cell
Corresponding Organization : University of California, San Diego
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Variable analysis
independent variables
- Immunohistochemistry analyses
- β cell mass
- β cell mass
- Microscopic images were analyzed using ImageJ software
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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