Proteasome activity was evaluated using yeast proteasome (TUM Groll group) in its storage buffer: 20 mM Tris/HCl pH 7.5. The inhibitory potency of the isolated compounds to yeast proteasome was assessed by using the Fluorescence Intensity Assay and Suc-Leu-Leu-Val-Tyr-AMC as substrate (Enzo Life Sciences, BML-P802-0005). The assay was carried out according to the method previously described [5 (link)]. An IC50 of 0.0366 nM was calculated for ONX-0914 using XLfit. It can generally be stated that the Fluorescence Intensity Assay worked properly, as the control compound ONX-0914, which was run in parallel to the assay, showed the expected results.
Assay BufferFluorescence Inhibitory Leu leu Onx 0914 Proteasome Tris Val tyr Yeast
Corresponding Organization :
Other organizations :
Écologie Marine Tropicale des Océans Pacifique et Indien, Aix-Marseille Université, Institut Méditerranéen de Biodiversité et d'Ecologie Marine et Continentale, Naturalis Biodiversity Center, National and Kapodistrian University of Athens, Institut de Chimie des Substances Naturelles
Inhibitory potency of the isolated compounds to yeast proteasome
control variables
Yeast proteasome (TUM Groll group) in its storage buffer: 20 mM Tris/HCl pH 7.5
Suc-Leu-Leu-Val-Tyr-AMC as substrate (Enzo Life Sciences, BML-P802-0005)
controls
Positive control: ONX-0914, which showed the expected results
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