Immunoblotting for Protein Analysis

Cells were treated with various drug concentrations, as indicated in the figure legends. Samples were isolated at the indicated times and SDS–PAGE and immunoblotting was performed as described in Booth et al. (2014a (link),b (link),c (link),d) (link). Immunoblots were observed by using an Odyssey IR imaging system (LI-COR Biosciences, Lincoln, NE).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Booth L., Roberts J.L., Tavallai M., Nourbakhsh A., Chuckalovcak J., Carter J., Poklepovic A, & Dent P. (2015). OSU‐03012 and Viagra Treatment Inhibits the Activity of Multiple Chaperone Proteins and Disrupts the Blood–Brain Barrier: Implications for Anti‐Cancer Therapies. Journal of Cellular Physiology, 230(8), 1982-1998.

Publication 2015

Odyssey IR imaging system

Cells Drug Immunoblots Sds page

Corresponding Organization : Virginia Commonwealth University

Other organizations : Bio-Rad (United States)

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Various drug concentrations

dependent variables

Protein expression levels (observed by immunoblotting)

control variables

Samples were isolated at the indicated times

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!