The screen was performed as previously described [58] (link), [85] (link). Except for the enzyme concentrations, all the rest was kept constant. The following assay concentrations were used. Thrombin (0.01 nM), α-chymotrypsin (0.03 nM), plasmin (0.8 nM), and chymase (0.45 nM) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Human skin β-tryptase (0.01 nM) was purchased from Promega (Madison, WI, USA), FXa (0.33 nM) from EMD Biosciences, Inc. (San Diego, CA, USA), FXIIa (0.1 nM) from Haematologic Technologies Inc. (Essex Junction, VT, USA), kallikrein (0.04 nM) from Fitzgerald Industries International (Concord, MA, USA), elastase (0.06 nM) from Elastin Products Company, Inc. (Owensville, MO, USA), and Cathepsin G (5.3 nM), FXIa (0.06 nM), urokinase plasminogen activator (uPA; 0.25 nM), and tissue plasminogen activator (t-PA; 0.02 nM) were purchased from Molecular Innovations (Southfield, MI, USA). Matriptase (0.03 nM) was obtained from R&D Systems (Minneapolis, MN, USA), proteinase 3 (11 nM) from Merck-Millipore (Billerica, MA, USA), and sequencing-grade trypsin (0.1 nM) was purchased from Roche Molecular Biochemicals (Indianapolis, IN, USA).
Human skin β-tryptase (0.01 nM) was purchased from Promega (Madison, WI, USA), FXa (0.33 nM) from EMD Biosciences, Inc. (San Diego, CA, USA), FXIIa (0.1 nM) from Haematologic Technologies Inc. (Essex Junction, VT, USA), kallikrein (0.04 nM) from Fitzgerald Industries International (Concord, MA, USA), elastase (0.06 nM) from Elastin Products Company, Inc. (Owensville, MO, USA), and Cathepsin G (5.3 nM), FXIa (0.06 nM), urokinase plasminogen activator (uPA; 0.25 nM), and tissue plasminogen activator (t-PA; 0.02 nM) were purchased from Molecular Innovations (Southfield, MI, USA). Matriptase (0.03 nM) was obtained from R&D Systems (Minneapolis, MN, USA), proteinase 3 (11 nM) from Merck-Millipore (Billerica, MA, USA), and sequencing-grade trypsin (0.1 nM) was purchased from Roche Molecular Biochemicals (Indianapolis, IN, USA).
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